Suppressive Effects of Anthriscus sylvestris Constituents on the Expression and Production of Matrix Metalloproteinase-9 Using Luciferase Transfected Raw 264.7 Cell Based Assay System

Suppressive Effects of Anthriscus sylvestris Constituents on the Expression and Production of Matrix Metalloproteinase-9 Using Luciferase Transfected Raw 264.7 Cell Based Assay System
Suppressive Effects of Anthriscus sylvestris Constituents on the Expression and Production of Matrix Metalloproteinase-9 Using Luciferase Transfected Raw 264.7 Cell Based Assay System

ㆍ 저자명: Lim. Hye-Sun,Oh. Sei-Ryang,Lee. Ho-Jae,Chin. Young-Won,Ahn. Kyung-Seop,Sok. Dai-Eun,Lee. Hyeong-Kyu
ㆍ 간행물명: Journal of applied biological chemistry
ㆍ 권/호정보: 2009년|52권 6호|pp.620-625 (6 pages)
ㆍ 발행정보: 한국응용생명화학회
ㆍ 파일정보: 정기간행물|ENG|
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ㆍ 주제분야: 기타

이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.

서지반출

기타언어초록

Bioactivity-guided fractionation of Anthricus sylvestris extract, using matrix metalloproteinase-9 (MMP-9) assay, led to the isolation of deoxypodophyllotoxin (1), falcarindiol (2), (-)-hinokinin (3), and (-)-hibalactone (4). All compounds obtained were evaluated for the inhibitory activities against MMP-9 in luciferase and zymographic assays. Of these compounds, compounds 1 ($IC_{50}$ 0.5 nM) and 2 ($IC_{50}8.4;{mu}M$) were found to inhibit MMP-9 expression in the PGL4.14-MMP-9-Luc plasmid transfected Raw 264.7 cells, and MMP-9 production in a gelatin zymographic assay.

키워드

Anthricus sylvestris deoxypodophyllotoxin falcarindiol luciferase assay matrix metalloproteinase-9 zymography

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