Chitosan is a natural non-toxic biopolymer and is widely used in various fields because of the antimicrobial activities. In this study, the properties of nylon textiles grafted with chitosan oligomer or chitosan polymer after being activated by open air plasma were evaluated. Results showed that nylon textiles grafted with chitosan polymer had better antibacterial performances than those grafted with chitosan oligomer. Air plasma activation at a higher speed (26 m/min) for a few times facilitated the grafting of chitosan and critically determined the antibacterial activities. Further treatment with air plasma after grafting improved the antibacterial effect. Overall, chitosan-grafted nylon textiles showed good antibacterial potential as well as biocompatibility.