This manuscript aimed at increasing the production of $alpha$-ketoglutarate by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019. The carbon flux was redistributed from pyruvate to $alpha$-ketoglutarate node by manipulating the specific activity of pyruvate dehydrogenase complex (PDH), pyruvate carboxylase (PC), pyruvate decarboxylase (PDC), and $alpha$-ketoglutarate dehydrogenase complex (KGDH). By proper increase of PDH, PC, and PDC activities, $alpha$-ketoglutarate in fermentation broth could be accumulated to the levels of 17.1 g/L, 21.6 g/L, and 31.2 g/L, respectively. In addition, decrease in the specific activity of KGDH also resulted in an enhanced $alpha$-ketoglutarate synthesis. With a proposed combinational enzymes regulation strategy, the highest $alpha$-ketoglutarate concentration of 37.7 g/L was achieved.