The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of $CH_2Cl_2$, BuOH, and $H_2O$ from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2"-acetylhyperoside with potent antioxidant effects against the DPPH radical, with $IC_{50}$ values of 1.31 and $7.09;{mu}g/mL$, respectively. They have not been reported from L. fischeri yet.