In this paper, the stability of second order uncertain systems with regulation of PID type controllers is analyzed by using Lyapunov second method for the first time in the time domain. The property of the stability of PID regulation servo systems is revealed in sense of Lyapunov, i.e., bounded stability due to the disturbances and uncertainties. By means of the results of this stability analysis, the maximum norm bound of the error from the output without variation of the uncertainties and disturbances is determined as a function of the gains of the PID control, which make it enable to analyze the effect resulted from the variations of the disturbances and uncertainties using this norm bound for given PID gains. Using the relationship of the error from the output without variation of the uncertainties and disturbances and the PID gain with maximum bounds of the disturbances and uncertainties, the robust gain design rule is suggested so that the error from the output without the variation of the disturbances and uncertainties can be guaranteed by the prescribed specifications as the advantages of this study. The usefulness of the proposed algorithm is verified through an illustrative example.