Purpose: The purpose of this study was to compare fatigue and fatigue-regulation behaviors in undergraduates courses related to public health (group A) and undergraduates in courses not related to public health (group B). Method: Using a structured questionnaire, data were collected from 236 undergraduates. Descriptive statistics, Pearson correlation coefficient, t-test with SAS package were used for data analysis, Results: There was a statistically significant difference between group A and group B in t-test comparison by group for fatigue, and all subcategories of fatigue and fatigue-regulation behaviors. The frequency of fatigue-regulation behavior of group A was 12.23 and the mean for total efficiency of fatigue-regulation behavior was 2.17, while the frequency of fatigue-regulation behavior for group B was 10.47 and the mean for total efficiency of fatigue-regulation behavior was 1.75. Finally, total fatigue and all subcategories of fatigue were positively related to fatigue-regulation behaviors. Conclusion: It is necessary to develop an intervention program for regulating fatigue in undergraduates courses related to public health.