The reproducibility of initial value and change over time of surface EMG spike variables(MSA, MSF, MSS, MSD) was investigated in the biceps brachii muscle of 11 healthy subjects. Surface EMG signals were recorded during sustained isometric voluntary contractions for 30 seconds at three contraction levels, 20%, 50%, and 80%MVC, respectively. Each contraction was repeated three times in each of three different days for a total of nine contractions and 99 contractions per %MVC level across the eleven subjects. A total of 297 EMG signals across the different trials, days, subjects, and %MVC levels was saved for the subsequent analysis. The degree of reproducibility was investigated using the intraclass correlation coefficient(ICC) and the standard error of the mean(SEM) based upon the analysis of variance(ANOVA), Results for intercept showed higher reproducibility of the spike variables with about 60%-98% ICC than the variable(ARV, MNF) which had been analyzed before in other researches. And results for slope showed poor reproducibility of the spike variables with about 30%-70% ICC and they were comparable with the variables of other researches.