This research is for dental hygienists to pursue affection and commitment in organization together with improvement of quality of working life by analyzing factors of working life affecting affective commitment in organization of dental hygienists, to find out plans to increase sense of accomplishments of working life and ultimate satisfaction of life, and to suggest direction for human resources management in management of dental clinics. Objects of investigation were dental hygienists working at dental medical institutions in Busan area with questionnaire survey and 197 sheets of questionnaires were used in the study. As the result of the study, high correlation was shown between composition elements of quality of working life and affective commitment per factor and as the result of quality factors of working life affecting affective commitment in organization, work environment, inter-personal relations, and social meaningfulness provided meaningful impacts on affective commitment in organization. It is believed to be important that dental medical institutions need to promote pride and love of companies in the future by providing regular social services or services for local residents so that employees can form smooth inter-personal relationships among employees with improvement of working environment and welfare in personnel management dimension. And for dental hygienists, they shall improve their work performances and enhance vocational values for development of their institutions and them with positive thinking through ceaseless efforts.