As RFID technology has been developed rapidly, its technical potential has it be widely used in many industrial fields. Particularly, in the physical distribution industry, the introduction of RFID has enormously contributed to effectively monitoring locations and information of products in real-time. Also, a significant decline in tag prices and RFID related technical competitiveness enabled each tag to be managed much more minutely by attaching it to an item, not a pallet nor a container. However, if a very large volume of tag data are continuously flowed into a RFID middleware with limited hardware resources, its entire data processing time may become considerably longer. Therefore, specific technologies are in great demand to handle and further to reduce the load of the middleware. In this paper, we proposed a mobile agent-based software module to efficiently reduce the load of the middleware by pre-processing a lot of tag data while items are in transit. Simulation results show that using the proposed software module considerably enhances the speed of processing tag data than otherwise. This behavior increases the tag recognition rate in a certain time limit and improves reliability of RFID middlewares.