Agroforestry combines agriculture and forestry technologies to create more integrated, diverse, productive, profitable, healthy and sustainable land-use systems. This study was performed in three union of Chhagalnaiya Upazila (Sub-district; administrative entity) under Feni district, Southern Bangladesh with a view to identify the tree resources, utilization pattern and economic return of major fruit and timber tree species. Information collected from a total of 45 households ranging from marginal, small, medium and large categories. Number of plant species increased with the increase of homestead area. A total of 39 plant species were recorded from the homegarden, of which 23 were fruit and 16 were timber tree species. Considerable number of vegetables was also planted under the shade of the homestead trees. The investment analysis showed that average benefit-cost ratios were greater than one, net present values were positive and internal rate of returns were more than 10%. Long term investment on horticulture and timber tree species is highly profitable if species like Swietenia mahagoni and Tectona grandis, Spondias pinnata, Syzygium cumini and Areca catechu were planted.