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Screening and Identification of a Streptomyces platensis YK-2, a New Transglutaminase Producer
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  • Screening and Identification of a Streptomyces platensis YK-2, a New Transglutaminase Producer
  • Screening and Identification of a Streptomyces platensis YK-2, a New Transglutaminase Producer
저자명
Yeo. Soo-Hwan,Yoon. Jung-Hoon,Lee. Dong-Gun,Kim. Hyun-Soo
간행물명
Journal of microbiology and biotechnology
권/호정보
2009년|19권 6호|pp.588-595 (8 pages)
발행정보
한국미생물생명공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A bacterial strain, YK-2, was isolated as a producer of trans glutaminase from a forest soil sample of Daegu, Korea. The isolate showed a G+C content of 72.7 mol%, contained meso-$A_2pm$ as the cell-wall amino acid, and possessed menaquinone MK-9 ($H_6$) and menaquinone MK-9 ($H_8$) at a ratio of 6:4. The chemotaxonomic analysis, as well as phylogenetic analysis based on the 16S rDNA sequence, identified the isolate as a member of Streptomyces platensis. For transglutaminase production, the optimum medium composition was determined to be 2% glucose, 1% polypeptone, 1% soy tone, and 0.1% $MnCl_2$. The transglutaminase was stable within the pH range of 5.0-9.0 and $30-45^{circ}C$, and the optimum pH and temperature were pH 8.0 and $45^{circ}C$, respectively, without any requirement for $Ca^{2+}$.