Force induced by the natural ventilation in tunnel is likely to generate adverse influences on the airflow during the normal operation and create even more unfavorable circumstances during the tunnel fire. The influence of the natural ventilation is required to take into account in designing and operating the ventilation as well as safety systems. The magnitude of natural ventilation force depends on a variety of factors associated with the topographical, meteorological and physical features of tunnel. Unfortunately, at this moment those are difficult to quantify and none of the countries has suggested its estimation method in the design guideline. This study aims at quantifying the natural ventilation force at a local highway tunnel by three different methods. The first method employes direct measurement of the pressure at portals, while the second applies a stepwise approach to eliminate the piston effect ahead of deriving the natural ventilation force and the third method uses the concept of barometric barrier.