The effect of the intake flow on the spray structure of a high pressure 11-hole fuel injector were examined in a single cylinder optical direct injection spark ignition (DISI) engine. The effects of injection timing and in-cylinder charge motion were investigated using the 2-dimensional Mie scattering technique. It was confirmed that in the homogeneous charge mode, the in-cylinder swirl charge motion played a major role in the fuel spray distribution during the induction stroke rather than the tumble flow. But, in the stratified charge mode, the effect of the in-cylinder charge was not so large that the injected spray pattern was nearly maintained and the increase of in-cylinder pressure by the upward moving piston reduced the fuel spray penetration.