This paper describes a new method for extracting characteristic impedance of transmission line using Substrate Integrated Artificial Dielectric (SIAD). The new procedure to calculate the characteristic impedance of SIAD transmission line is described with the proper equations and basic transmission line theory. The characteristic impedance is determined as an almost fixed value in the proposed method, while it fluctuates according to frequency in the previous method. As the result, the effective dielectric constant of SIAD transmission line is calculated as a fixed value rather than fluctuating one. In order to show the validity of the proposed method, SIAD microstrip lines are simulated and measured, and characteristic impedances and effective dielectric constant are calculated and compared to the previous method.