Objectives : The purpose of this study was to investigate the relation between neck pain and physical factors in female office workers. Methods : Neck pain group of 31 female subjects complained of neck and arm discomfort. Normal group of 20 female subjects had no complaints or minimal discomfort. Cervical curvature and muscle tone were assessed by whole spine x-ray, meridian-electromyography(MEMG), craniovertebral angle. Neck pain was evaluated by Neck Disability Index(NDI) and Visual Analog Scale(VAS). Results : The NDI score and contraction power of upper trapezius by MEMG had a relationship significantly. However, there was no relationship between NDI and cervical curvature. Conclusions : The results suggest that neck pain is related to muscle tone rather than physical stress and cervical curvature.