Purpose : This study aims to comparison of ultrasonography on both sides of biceps brachii muscle of hemiplegic patient. Methods : The biceps brachii muscle of fifteen subjects (5 male, 10 female) with stroke were scanned with ultrasonography at the muscle belly at rest at elbow angles of 90 deg. The echogenicity (density, white area index; WAI, anatomic cross-sectional area; ACSA, volume) of ultrasonography was examined. Results : In the biceps brachii muscle, there were significant differences of density, WAI, ACSA, and volume between affected side and non-affected side. biceps brachii muscle, echogenicity (density, WAI) of the affected side was higher than non-affected side. biceps brachii muscle, echogenicity (ACSA, volume) of the non-affected side was higher than affected side. Conclusion : This study showed that change of skeletal muscle architecture properties occurred biceps brachii muscle of the affected side muscle of hemiplegic patient.