Unlike concrete bridge, steel bridge resists external force by forming thin plate. Thus, because steel girder bridge has big slenderness ratio, buckling is a major design factor. Plate girder consists of flange and web plate. Because of economic views, web plate that resists shear forces is made by more thinner plate. Thus, web plate has much risk for buckling. The objective of this study is to analyze the buckling behaviors of plate girder and to present the proper reinforcement location of longitudinal stiffeners. Various parametric study according to the change of web height, transverse stiffeners and load condition are examined.