The performance of anti-collision algorithm in RFID systems, which are based on FSA algorithm, may be affected by the frame size a query round. In this paper, an anti-collision algorithm with early cancellation of query round is proposed to enhance the performance of EPCglobal Class-1 Gen-2. The Q-algorithm calculates a Q value to determine the next frame size during a query round. In the proposed algorithm, if the new Q value is different from the previous one, the reader transmits a QueryAdjust command to cancel the current query round. The simulation results show that the proposed algorithm can have a stable performance irrespective of the C value of Q-algorithm and the number of tags.