This study has intended to build ecological networks in consideration of life-zones inside Cheongju city through biotope grade, GIS network analysis etc. This study consisted of following three steps. First, we selected core districts and core spot districts using land use patten and biotope grade. The core district included the first grade of biotope and forest land. The core district consisted of two sectors : east axis core, Uam mountain; west axis core, Bumo mountain. The core spot district included the first grade of biotope. The core spot districts consisted of two sectors : north axis base core, Myongshim park; south axis base core, Guryong park. Second, the base district included the second grade of biotope and park and school. We used buffering analysis within 500m of the base district and selected the new base district. Third, we connected core districts and base core districts using least cost analysis of GIS. Thus we built comprehensive ecological networks in consideration of life-zones through GIS.