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Selective Plugging Strategy Based Microbial Enhanced Oil Recovery Using Bacillus licheniformis TT33
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  • Selective Plugging Strategy Based Microbial Enhanced Oil Recovery Using Bacillus licheniformis TT33
저자명
Suthar. Harish,Hingurao. Krushi,Desai. Anjana,Nerurkar. Anuradha
간행물명
Journal of microbiology and biotechnology
권/호정보
2009년|19권 10호|pp.1230-1237 (8 pages)
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한국미생물생명공학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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The selective plugging strategy of Microbial Enhanced Oil Recovery (MEOR) involves the use of microbes that grow and produce exopolymeric substances, which block the high permeability zones of an oil reservoir, thus allowing the water to flow through the low permeability zones leading to increase in oil recovery. Bacillus licheniformis TT33, a hot water spring isolate, is facultatively anaerobic, halotolerant, and thermotolerant. It produces EPS as well as biosurfactant and has a biofilm-forming ability. The viscosity of its cell-free supernatant is $120;mPa{cdot}s$ at $28^{circ}C$. Its purified EPS contained 26% carbohydrate and 3% protein. Its biosurfactant reduced the surface tension of water from 72 to 34 mN/m. This strain gave $27.7{pm}3.5%$ oil recovery in a sand pack column. Environmental scanning electron microscopy analysis showed bacterial growth and biofilm formation in the sand pack. Biochemical tests and Amplified Ribosomal DNA Restriction Analysis confirmed that the oil recovery obtained in the sand pack column was due to Bacillus licheniformis TT33.