The primary purposes for using fire are to enhance marsh vegetation to support waterfowl, and to manage invasive plant species. The study was conducted for two consecutive years in 2004 and 2005, investigating the effects of prescribed fire regimes on vegetation biomass in tidal brackish marsh areas of the Blackwater National Wildlife Refuge located on the eastern shore of Maryland, USA, that are under relatively similar environmental conditions. Four different burn regimes (i.e., annual burn, 3-5 year burn, 7-10 year burn, and no burn) were applied in the study. Above- and below-ground vegetation biomass samples as affected by the different burn regimes were harvested in each year for five plant species native to the marsh; Distichlis spicata, Spartina alterniflora, Schoenoplectus americanus, Spartina cynosuroides and Spartina patens. No significant difference was found either in total above-ground biomass or in above-ground biomass by species between burn regimes in 2004. However, more total above-ground biomass was produced in annual burn regime in 2005 than in the other burn regimes. There were no consistent effects of burning on vegetative biomass production by species, but it seemed D. spicata was somewhat benefited by prescribed burning for its biomass production. Moreover, the stem density for D. spicata under annual burn regime was significantly higher than that in the other burn regimes, showing some positive effects of burning on vegetation. The below-ground biomass was significantly greater in 2004 than in 2005, yet with no significant difference between burn regimes in either year. A longer-term monitoring is strongly recommended.