Micro particles in fluid can be manipulated by using ultrasonic standing wave since the ultrasound makes particles move by means of its acoustic radiation force. This work concerns the micro particle manipulation system using ultrasonic standing wave which consists of a microchannel, a reflector, and an ultrasonic transduer. In the present system, the effects of the structural elements should be carefully considered to comprehend the system and find the optimal operational condition. In this investigation, finite element analysis was employed to analyze the system. Some interesting characteristics on the reflector thickness, the channel width, and the operational frequency were observed. Several experimental results were compared with the analytic results. Consequently, this work solidifies the importance of those system parameters and reveals the possibility of various applications of the particle manipulation using ultrasonic standing wave.