본 연구는 고지방식이로 비만을 유도한 흰쥐에 4주간 마늘진액섭취와 treadmill running을 실시한 후 탈진적 운동을 실시하여 체중, 지방량, 혈중지질 및 산화적 스트레스에 미치는 영향에 미치는 영향을 조사하였다. 실험대상은 3주령 SD계 rats 30마리를 6주간 고지방식이로 비만을 유도한 후 정상식이군(N), 고지방식이군(HD), 운동군(E), 운동과 마늘섭취군(EA), 마늘섭취군(A)으로 구분하였고, 4주간 treadmill running과 운동 30분 전에 AGE를 섭취시킨 후 실험최종일 모든 그룹을 고강도 탈진적 운동을 실시하였다. 그 결과 6주간 고지방식이로 비만을 유도한 고지방식이군(HD)은 정상식이군(N)에 비해 체중과 체중 증가량이 유의하게 높게 나타났다(p<0.001). 그리고 4주간 treadmill running과 마늘섭취를 병행한 결과, 운동군(E)과 운동과 마늘섭취군(EA)에서 체중 증가량, 복부지방량과 부고환지방량이 다른 군에 비해 유의하게 높게 나타났으며(p<0.05), 비복근과 가자미근은 그룹 간 유의한 차이가 나타나지 않았다. 혈중지질의 변화에서 그룹 간 유의한 차이가 나타나지 않았고, 산화적 스트레스는 운동군(E)보다 마늘섭취(A)군이 유의하게 낮게 나타났다(p<0.05). 따라서 규칙적인 유산소 운동과 마늘진액섭취는 체중과 체지방량 감소에 유의한 효과를 나타내고, 마늘진액섭취가 규칙적인 유산소 운동보다 효과적으로 산화적 스트레스를 방어하는 것으로 사료된다.
The purpose of this present study was to investigate the effects of long-term treadmill running and aged garlic extract supplementation on weight, adipose tissue mass, lipid profiles and oxidative stress after exhaustion in high fat diet-induced obese rats. Thirty male Sprague-Dawley rats (3 weeks old) were used as subjects. High fat diets were fed to all subjects for 6 weeks in order to induce obesity. The subjects were divided into five groups - N (normal diet group), HD (high fat diet group), E (exercise group), EA (exercise with AGE diet group) and A (AGE diet group). Aerobic exercise was performed through treadmill running and AGE at a dosage of 2.86 kg/g was administered to rats 30 min before every exercise for 4 weeks. After 4 weeks, all groups completed acute treadmill running (speed increasing gradually to 25 m/min, 15% uphill grade) until exhaustion. Immediately after exhaustive treadmill exercise, the weight, adipose tissue mass, lipid profiles and oxidative stress of the rats were assayed. At the end of 6 weeks of high fat diets, body weight and body weight gain were significantly higher in the high fat diet groups than in the normal diet group (p<0.001). At the end of treadmill exercise with AGE intake for 4 weeks, body weight gain, visceral and epididymal fat of the E and EA groups were significantly decreased compared to other groups (p<0.05). There were no significant differences in gastrocnemius and soleus. T-C, HDL-C, TG and LDL-C were not significant in any of the groups. TBARS was significantly lower in the A group than in the E group (p<0.05). These results indicated that body weight gain, visceral and epididymal fat decreased in the E and EA groups, and TBARS levels were lower in the A group than the E group. Regular aerobic exercise intervention with AGE supplementation may also modify the adipose weight and improve the oxidant stress in obese rats.
