Adult chironomids were collected at Dangsan-ri, Muju-eup, Muju-gun, Jeollabuk-do on 5 September 2008, 22 May 2009 and 28 August 2009. A total of 221 specimens belong to the genus Polypedilum Kieffer from 1,113 adult midges collected were morphologically examined, and 16 Polypedilum species were identified. One species is new (Polypedilum dangsanensis Ree et Jeong sp. nov.) and eight species are newly recorded in Korea (P. asakawasense, P. convictum, P. decematogutatus, P. japonicum, P. kamotertium, P. pullum, P. serugense, and P. unifascium). These nine species are described with illustrations. Polypedilum nubifer was the most frequently collected species, consisting of 25.8% of the Polypedilum samples.