Purpose : The purpose of this study was to investigate knee muscle strength, balance, and proprioception by walking while giving weight to the ankle joint at a load of 0%, 10%, and 15% gradient on a treadmill. Methods : Twenty-four subjects were divided into three groups. Group A walked on a 0% gradient while giving weight to the ankle joint as a load. Group B walked on a 10% gradient while giving weight to the ankle joint as a load. Group C walked on a 15% gradient while giving weight to the ankle joint as a load. This was performed three times per week for four weeks. The walking speed was set at 4km/hour and the walking time was set at 20 minutes. Measurements were performed before and after the experiment. Biodex medical system and Balance system SD were used as measuring instruments. Results : There was no significant difference between the gradients in muscle strength, balance and proprioception. However, there were differences in pretest and posttest measurements for muscle strength, balance and proprioception in each gradient. In particular, the gradient of 10% showed a significant difference. Conclusion : According to the results of this study, to use a 10% gradient is effective when walking while giving weight to the ankle joint as a load.