The ultra high performance liquid chromatography (u-HPLC) method for determination of $eta$-carotene in foods was validated in terms of precision, accuracy, and linearity. The u-HPLC separation was performed on a reversed column $C_{18}$ (particle size 2 ${mu}m$, i.d. 2 mm, length 50 mm), followed by ultra violet (UV) detection at 450 nm. The recovery of $eta$-carotene was more than 84.4% and the limit of detection and limit of quantitation of u-HPLC analysis were 0.28 and 0.85 ${mu}g$/mL for $eta$-carotene with butylated hydroxytoluene (BHT) and 0.62 and 1.89 ${mu}g$/mL for $eta$-carotene without BHT, respectively. The calibration graph for $eta$-carotene was linear from 0.1 to 25.0 ${mu}g$/mL for u-HPLC. The intra- and interday precisions (relative standard deviations) were <7.5 and <7.8%, respectively. Benefits of u-HPLC analysis of $eta$-carotene in foods is reduction of the analysis time to approximately 1/4, saving the volume of solvent to approximately 1/15. It seems that u-HPLC can offer significant improvements in speed, sensitivity, and resolution compared with conventional HPLC, this bodes well for future applications.