This study demonstrated that cryopreserved semen from dead fish can be used for seedling production. Yellow croakers, Larimichthys polyactis, were killed and stored at temperatures of $20^{circ}C$ or $0^{circ}C$ for 6 hours. At 2 hour intervals, semen from these fish was collected using abdominal pressure and evaluated for spermatozoa motility and semen cryopreservation. Semen collected after 6 hours from dead fish stored at $0^{circ}C$ could be cryopreserved and attained fertilization and hatching rates of $15.0{pm}1.2%$ and $14.8{pm}1.6%$, respectively. This study suggests that germ cells such as the semen of dead fish can be cryopreserved and utilized in the restoration of a species.