The present study was performed on humans to investigate the physiological strain of wearing protective clothing for shielding electromagnetic waves and to compare control clothing that are currently on the market and new clothing that are developed for improving thermal comfort and material weight. Experiments were conducted in a climatic chamber of $28.8{pm}0.6^{circ}C$, $37{pm}5%$RH under three differed experimental clothing conditions: None, Control, New. The results were as follows. Mean skin temperature and rectal temperature in New were significantly lower than that in None and Control (p<.05). The temperature and humidity inside clothing were lower in None (p<.05). Total weight loss was lower in New. Thermal sensation and thermal comfort were less hot and more comfortable in New than those in Control. It was concluded that wearing the protective clothing for shielding electromagnetic waves affects physiological responses such as distribution of body temperature, sweat rate, etc.