A fuel injection system using an ECU HILS as an alternate to a vehicle test for the fuel injectors was developed. The throttle position, vehicle speed, engine speed, crank position, cam position, intake air flow, and several other sensor signals that are supplied to the ECU were measured and recorded as a data file for a vehicle driven in the FTP-75 mode in a chassis dynamometer. Electric signals that are equivalent to the sensor signals from the vehicle are reconstructed from the recorded data file using data acquisition boards, microprocessors, and computers. All sensor signals are supplied to the ECU with synchronized timing using a computer program. The findings show that the cost and time of vehicle experiments can be reduced using the ECU HILS system. Moreover, the repeatability of the generation of sensor signals can enhance the accuracy of a range of experiment related to vehicle testing. An ECU scanner that scans the sensor signals that are input to the ECU through a serial port was used to assess the accuracy of the reconstructed signals. The scanning results show good agreement with the reconstructed input signals. Injectors were connected to the ECU HILS system and were driven by the system to measure the quantity of injected fuel.