The purposes of this study is to verify the fracture characteristic of steel which is manufactured in Korea, subjected to cyclic loading. This investigation deals with the low cycle fatigue behavior of longitudinal reinforcement in reinforced concrete bridge substructure (piles and columns of piers). Eighty-one specimens of longitudinal reinforcement were tested under axial strain controlled reversed cyclic tests with strain amplitudes. The selected test variables are ratio of tension strain to compression strain, yield stress of longitudinal reinforcement, ratio of diameter of longitudinal steel to clear length of longitudinal steel, size of longitudinal steel and strain amplitudes. Low cycle fatigue behavior and low-cycle fatigue life are investigated and discussed in this paper.