In this study, we propose a novel method to detect and enhance the feature of ST-segment which offers the crucial information for the diagnosis of myocardial infarction and ischemia. With this aim, PQRST features of Electrocardiogram initially are detected and subsequently ST-segment are estimated. And Dynamic Time Warping(DTW) transformation is applied recursively to minimize the difference in time between ST-segments and calculate the minimum cumulative distance that decides the degree of similarity among ST-segments. As of the results, the inherent characteristic of ST-segment can be emphasized in terms of time parameter and thus the diagnostic features of a ST-segment can be revealed further.