An efficient protocol was established to produce virus free in vitro banana plantlets through meristem culture of virus infected four months old banana suckers cv. Sabri. Murashige and Skoog (MS) medium supplemented with different levels of BAP (0, 3, 4 and 5 $mg{cdot}L^{-1}$) and IAA (0.0, 1.0, 1.5, 2.0 and 2.5 $mg{cdot}L^{-1}$) were used for shoot proliferation in Experiment-I. Half strength of MS medium supplemented with different levels of IBA (0, 1, 2, 3 $mg{cdot}L^{-1}$), NAA (0, 2, 3, 4 $mg{cdot}L^{-1}$) were treated for root formation in Experiment-II. ELISA test was performed to confirm virus eradication rate of regenerated plants and survival rate of regenerated plants in Experiment-III. The treatment combination of 4 $mg{cdot}L^{-1}$ BAP + 2.5 $mg{cdot}L^{-1}$ IAA produced the highest number of shoots (3.4 $explant^{-1}$), while the longest shoot (3.49 cm), maximum number of leaves (3.37 $plantlet^{-1}$) and longest leaves (2.22 cm) were obtained in the culture treated with 5 $mg{cdot}L^{-1}$ BAP. On the contrary, for plantlets cultured on half strength of MS medium supplemented with different levels of IBA and NAA, the highest number of vigorous roots (8.01 $explant^{-1}$) and maximum root length (4.64 cm) were observed at treatment combinations of 2 $mg{cdot}L^{-1}$ IBA + 4 $mg{cdot}L^{-1}$ NAA and 2 $mg{cdot}L^{-1}$ IBA+ 3 $mg{cdot}L^{-1}$ NAA, respectively. The combinations of 3 $mg{cdot}L^{-1}$ IBA + 3 $mg{cdot}L^{-1}$ NAA produced more vigorous roots while less vigorous roots were observed at respective control. The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected sources was 57.14%. However, the highest plant survival rate (83.33%) was observed in a mixture of sand, soil and cow dung (1:1:1) and in a mixture of coir and soil (1:2). These results suggested that eradication of BBTV through meristem culture can be used as an effective tool for commercial exploitation of virus free banana production.