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Somatic Embryogenesis in Cyclamen with Two Explants and Combinations of Plant Growth Regulators
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  • Somatic Embryogenesis in Cyclamen with Two Explants and Combinations of Plant Growth Regulators
  • Somatic Embryogenesis in Cyclamen with Two Explants and Combinations of Plant Growth Regulators
저자명
Jalali. Neda,Naderi. Roohangiz,Babalar. Mesbah,Mirmasoumi. Masoud
간행물명
Horticulture, Environment, and Biotechnology
권/호정보
2010년|51권 5호|pp.445-448 (4 pages)
발행정보
한국원예학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

$Cyclamen$ $persicum$ Mill. (Myrsinaceae ) is one of the most important winter pot flowers in the world. It is commercially propagated via $F_1$-hybrid seeds. Somatic embryogenesis is an efficient method of micropropagation and biotechnological tools for genetic improvement in cyclamen. The effect of initial explants type (leaf and petiole) on callus growth and somatic embryo were studied. Somatic embryos were cultured on modified Murashige and Skoog (MS) medium. Also embryogenic callus was induced on several media containing 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin). The greatest amount of callus was observed in two explants on media containing 1 $mg{cdot}L^{-1}$ 2,4-D + 0.5 $mg{cdot}L^{-1}$ Kin. The optimal responses of somatic emberyogenesis were recorded on media enriched with 4 $mg{cdot}L^{-1}$ 2,4-D, 0.1 $mg{cdot}L^{-1}$ Kin and leaf explants. These results were obtained from adult plants.