유기성 폐기물인 음식물쓰레기를 이용하여 유용한 에너지원인 바이오에탄올을 생산하고자 하였으며, 에탄올 생산 균주는 Saccharomyces cerevisiae를 이용하였다. 음식물쓰레기의 당화를 위하여 carbohydrase와 glucoamylase 효소를 이용한 결과 carbohydrase가 glucoamylase보다 당화효율이 우수하였으며, carbohydrase 이용시 건조 음식물쓰레기 기준 glucose 생산량 0.63 g/g-TS을 얻을 수 있었다. 에탄올 생산은 동시당화발효에서 0.44 g/$L{cdot}hr$, 분리당화발효가 0.27 g/$L{cdot}hr$이었다.
In the present study, bioethanol was produced using batch style reactor from food wastes which has organic characteristics. Pretreatment was required to reduce its particle size and produce fermentable sugar. Two different enzymes such as carbohydrase and gulcoamylase were tested for saccharification of food waste. The efficiency of carbohydrase saccharification (0.63 g/g-TS) has shown higher than glucoamylase saccharification(0.42 g/g-TS). Saccharomyces cerevisiae produced bioethanol via separate hydrolysis & fermentation (SHF) method and simultaneous saccharification fermentation (SSF) method. The production amount of bioethanol was 0.27 g/$L{cdot}hr$ for SHF and 0.44 g/$L{cdot}hr$ for SSF.
