The vibration of fungal cultures was evaluated to determine its potential effect on the dispersal of airborne fungal microorganisms suspected of being pathogens. An artificial vibration system, which simulates the actual environmental vibration of fungal structures, was designed and constructed for this purpose. Experiments featured the use of low-frequency vibrations similar to those induced by earthquakes. Within the range of conditions tested, the vibration of fungal cultures was found to affect the airflow-driven generation of bioaerosols.