Compositional changes in ginsenosides and crude saponin during enzymatic biotransformation of red ginseng were studied on three commercial polysaccharide-hydrolyzing enzymes, Pectinex, Termamyl, and Celluclast. Considerable enhancement of crude saponin production was observed with Pectinex, whereas hydrothermally processed black ginseng showed significant decrease. Celluclast-treated red ginseng contained five-fold higher concentration of protopanaxadiol-type ginsenoside Rd, with decrease of precursor Rb1. Red ginseng treated with Pectinex showed distinct increases of protopanaxatriol-type ginsenosides Re and Rg1.