Vaccines, especially for viruses, have been produced from egg-based manufacturing process. The method is simple and easy to set up the manufacturing process. However, the method has many problems in quality control, limit of manufacturing capacity, and ethical issues. Over the last decade, an alternative method, which manufactures vaccines using cell culture-based system, has received great attention to overcome the problems in egg-based vaccine production. This article examines current status and perspectives of cell culture-based vaccine production.