The purpose of this study is to investigate the antioxidative activity and digestive enzyme inhibition of grape seed extract (GSE). The GSE was tested for its effect on various antioxidative potentials (scavenging activities of DPPH radical, superoxide anion radical and hydroxyl radical) and inhibitory effect of various digestive enzymes (trypsin, $alpha$-chymotrypsin, $alpha$-amylase, $eta$-glucosidase and lipase). DPPH radical scavenging activity ($SC_{50}$, 50% scavenging concentration) of GSE was 4.76${pm}$0.27 ppm while those of positive controls (EGCG and vitamin C) were 2.22${pm}$0.12 ppm and 9.50${pm}$0.72 ppm, respectively. $SC_{50}$ value of GSE against superoxide anion radical and hydroxyl radical were 3.82${pm}$0.07 ppm and 803.23${pm}$27.16 ppm, respectively. In addition, $IC_{50}$ values of GSE against trypsin, $alpha$-chymotrypsin, $alpha$-amylase, $alpha$-glucosidase and lipase were 2.17${pm}$0.59 ppm, 7.46${pm}$1.25 ppm, 18.25${pm}$3.54 ppm, 12.30${pm}$1.12 ppm, and 653.23${pm}$79.34 ppm, respectively. These results suggest that GSE may be useful for the prevention or treatment of obesity.