The development of fungal biopesticides requires the efficient production of large numbers spores or other propagules. The current study used published information concerning carbon concentrations and C:N ratios to evaluate the effects of carbon and nitrogen sources on sporulation of Paecilomyces lilacinus (IPC-P and M-14) and Metarhizium anisopliae (SQZ-1-21 and RS-4-1) in a two-stage cultivation system. For P. lilacinus IPC-P, the optimal sporulation medium contained urea as the nitrogen source, dextrin as the carbon source at 1 g/L, a C:N ratio of 5:1, with $ZnSO_4{cdot}7H_2O$ at 10 mg/L and $CaCl_2$ at 3 g/L. The optimal sporulation medium for P. lilacinus M-14 contained soy peptone as the nitrogen source and maltose as the carbon source at 2 g/L, a C:N ratio of 10:1, with $ZnSO_4{cdot}7H_2O$ at 250 mg/L, $CuSO_4{cdot}5H_2O$ at 10 mg/L, $H_3BO_4$ at 5 mg/L, and $Na_2MoO_4{cdot}2H_2O$ at 5 mg/L. The optimum sporulation medium for M. anisopliae SQZ-1-21 contained urea as the nitrogen source, sucrose as the carbon source at 16 g/ L, a C:N ratio of 80:1, with $ZnSO_4{cdot}7H_2O$ at 50 mg/L, $CuSO_4{cdot}5H_2O$ at 50 mg/L, $H_3BO_4$ at 5 mg/L, and $MnSO_4{cdot}H_2O$ at 10 mg/L. The optimum sporulation medium for M. anisopliae RS-4-1 contained soy peptone as the nitrogen source, sucrose as the carbon source at 4 g/L, a C:N ratio of 5:1, with $ZnSO_4{cdot}7H_2O$ at 50 mg/L and $H_3BO_4$ at 50 mg/L. All sporulation media contained 17 g/L agar. While these results were empirically derived, they provide a first step toward low-cost mass production of these biocontrol agents.