A novel manganese peroxidase of Rhizoctonia sp. SYBC-M3 (R-MnP) was purified by $(NH_4)_2SO_4$ fractionation, DEAE-cellulose-32 column chromatography, and Sephadex G100 column chromatography. The molecular mass of R-MnP was determined to be approximately 40.4 kDa by SDS-PAGE. The optimum temperature and pH for R-MnP were $55^{circ}C$ and 4.5, respectively. R-MnP was highly stability when the temperature was below $50^{circ}C$. R-MnP could retain about 60% of its activity when the pH was between 4 and 6.5. However, R-MnP activity was inhibited by $Fe^{3+}$, $Cu^{2+}$, and $Co^{3+}$ as well as increased by $Zn^{2+}$ and $Ca^{2+}$. R-MnP demonstrated oxidation of DMP, ABTS, veratryl alcohol, and guaiacol. The $K_m$ values of RMnP for $H_2O_2$ and $Mn^{2+}$ were 25.3 and 53.9 ${mu}$mol/L, respectively.