In vitiligo, active melanocytes in the epidermis are not present, whereas melanoblast cells in the outer root sheath of hair follicles are not affected. The existence of these inactive melanocytes provides a source for repigmentation of vitiligo. To evaluate the potential of herbal candidates in treatment of vitiligo, we studied the effect of Cassia alata leaf extract on the differentiation, proliferation and migration of melanoblast cells. Melanin content increased in melb-a melanoblast cells in response to this herb extract in a dose-dependent manner compared to control cells. In addition, it induced tyrosinase activity and altered melb-a cell morphology. A transwell migration assay showed the potential of this herbal candidate to induce direct migration of treated cells. To our knowledge, this is the first report on activity of this kind in Cassia alata. The findings of the present study are significant in the direction of developing safer strategies for vitiligo treatment.