Bacterial cellulose is formed by microbial Gluconacetobacter xylinus, which produces a cellulose pellicle in its culture medium. The bacterium Gluconacetobacter sp. V6 was cultured in three types of media using an agitation method: a standard Hestrin-Schramm medium and a medium modified with either glycerol or molasses as the carbon sources. The structural properties of the cultured bacterial cellulose, such as crystallinity, viscosity and mechanical properties, were examined. Cell growth was excellent in the agitated culture with the molasses medium, and the cellulose yield was excellent in the agitated culture with the glycerol medium. The agitated culture with the molasses medium resulted in increased cell growth and cellulose yield, as well as improved mechanical properties of cellulose compared to the static culture with the molasses medium.