Cu and Zn can be accumulated in plastic film house soils by long-term application of livestock manure or compost. The mobility and bioavailability of Cu and Zn accumulated in soils are strongly influenced by their chemical or geochemical species in soils. In order to assess the accumulation, mobility, and bioavailability of Cu and Zn in plastic film house soils, we determined their geochemical species using a sequential extraction, grouped into three pods: the total pool, the potentially mobil pool, and the mobil pool. Total contents of Cu and Zn, ranged from 14.9 to 53.1 mg $kg^{-1}$ for Cu and from 55.4 to 169 mg $kg^{-1}$ for Zn, lied by far below the soil contamination standards, but exhibited little accumulation compared with their geogenic concentrations. Mobile contents of Cu and Zn and their percentage of total contents were strongly affected by soil pH in addition to total contents and soil organic matter. Mobile contents of Cu, ranged from <0.01 to 1.71 mg $kg^{-1}$, showed their minimum between pH 5.0 and 6.0 and increased above pH 6.0 to 8.0. In contrast, mobile contents of Zn, varied from <0.01 to 12.4 mg $kg^{-1}$, showed their minimum above pH 7.0 and increased strongly with decreasing pH below 5.5~6.0. Potentially mobile and total contents of Cu and Zn rose with ascending soil organic matter. To assess ecological and toxic effects of Cu and Zn in soils, mobile and potentially mobile contents, as bioavailable and potentially bioavailable pools, should be considered more important than total contents.