This paper presents finite element analyses, experimental measurements and finite element model updating of an arch type steel laboratory bridge model using semi-rigid connections. The laboratory bridge model is a single span and fixed base structure with a length of 6.1 m and width of 1.1m. The height of the bridge column is 0.85 m and the maximum arch height is 0.95 m. Firstly, a finite element model of the bridge is created in SAP2000 program and analytical dynamic characteristics such as natural frequencies and mode shapes are determined. Then, experimental measurements using ambient vibration tests are performed and dynamic characteristics (natural frequencies, mode shapes and damping ratios) are obtained. Ambient vibration tests are performed under natural excitations such as wind and small impact effects. The Enhanced Frequency Domain Decomposition method in the frequency domain and the Stochastic Subspace Identification method in the time domain are used to extract the dynamic characteristics. Then the finite element model of the bridge is updated using linear elastic rotational springs in the supports and structural element connections to minimize the differences between analytically and experimentally estimated dynamic characteristics. At the end of the study, maximum differences in the natural frequencies are reduced on average from 47% to 2.6%. It is seen that there is a good agreement between analytical and experimental results after finite element model updating. Also, connection percentages of the all structural elements to joints are determined depending on the rotational spring stiffness.