Purpose: This study was performed to identify the relationship between Sense of Humor, Coping Humor, Perceived stress and self-esteem in the middle-aged women. Methods: Data were collected from the 20th of June to the 30th of August in 2009 from 145 middle-aged women living in Seoul. Data analysis was done with SPSS/WIN 12.0 program for descriptive statistics and Pearson correlation. Results: The mean score of the humor sense was $55.94{pm}6.52$, coping humor was $21.11{pm}3.82$, perceived stress was $22.21{pm}3.03$ and self-esteem was $27.50{pm}2.88$. Subcategories of sense of humor were that emotional expressiveness was average $20.75{pm}2.70$, meta-message sensitivity was average $20.12{pm}2.74$ and liking of humor was average $15.13{pm}3.44$. Sense of humor was difference according to the economic status(t=2.000, p=.047), coping humor was difference according to the on medication use(t=2.227, p=.027). There was a significant correlation between the coping humor, perceived stress and the self-esteem. When the coping humor was higher, perceived stress was lower and the self-esteem was higher. Conclusion: This study showed that medication and economic status influences the humor scores and the sense of humor and coping humor were related to perceived stress and self-esteem in middle-aged women Therefore, these findings can provide the basis for the development of nursing intervention to increase the ability to coping with stress.