Evaluation of uncertainty is an ongoing process that can consume time and resources. It can also require the service of someone who is familiar with data analysis techniques. Therefore, it is important for laboratory personnel who are approaching uncertainty analysis for the first time to be aware of the resources required. International inclination of measurement filed to guarantee the traceability and confidence of measurement results discards the error concept and instead analyzes the measurement uncertainty. In this paper, we analyzed the elements of measurement uncertainty on surface roughness test which are the important things in mechanical parts test. Repeat the test by 3 men, the measurement uncertainty could be calculated.