This study examined the encapsulation of submicron-sized silica particles by stimuli-responsive copolymershell layer. Silica/poly(2-dimethylaminoethyl methacrylate-N-isopropylacrylamide-ethyleneglycol dimethacrylate), abbreviated as silica/P(DM-NIPAM-EGDM) core-shell composite polymer particles, were prepared by the seeded copolymerization of DM, NIPAM and EGDM in the presence of silicaparticles. The morphology and size distribution were analyzed by scanning electron microscopy. The surface composition showed silicaparticles covered with a copolymer shell layer consisting of P(DM-NIPAM-EGDM). The temperature and pH dependent adsorption behavior of the low molecular weight surfaetants and biomolecules on the silica/P(DM-NIPAM-EGDM) composite polymer particles indicated a stimuli-responsive phase transition. The activity of the adsorbed trypsin (TR) on the composite particles remained highcompared to the free TR and adsorbed TR on silicaparticles.