This study was carried out to investigate the optimum condition for sap exudation of Acer mono Max. tree in a site of Mt. Baekun, Kwangyang city, Korea. Amount of sap exudation, air temperature, relative air humidity and tree diameter at breast height (DBH) were monitored for the period of January 5 through March 28, 2008, and correlation analysis of several factors affecting on sap exudation was carried out. As the diameter of Acer mono at breast height increased, the amount of sap was linearly proportional. Sap exudation initiated at February 18, and occurred intensively in the period of February 28 through March 10, resulting in 84% of total sap amount by volume. During sap exudation, the minimum temperature was averaged at $-2.4{pm}1.5^{circ}C$ and the maximum at $6.0{pm}1.8^{circ}C$, while there was no sap exudation whenever temperature was below or above $0^{circ}C$ all the day long. The maximum temperature, range of temperature and the maximum, minimum and mean humidities in air were significant factors affecting on amount of sap. The maximum air temperature had the highest correlation coefficient with 0.768 (P < 0.01) and was also considered as the principal factor by partial-correlation analysis. These results showed that sap exudation required daily air-temperature fluctuation from below to above $0^{circ}C$, and the amount of sap was strongly dependent on the highest daily-temperature and DBH of tree.