In this study, a gelatin conjugated $Pluronic^{(R)}$ thermosensitive polymer(Gelatin-Pluronic, GP) was synthesized by the direct coupling of gelatin to monocarboxylated $Pluronic^{(R)}$(MP) and characterized. The use of thermosensitive polymers has recently been proposed for many applications including tissue engineering, gene therapy, novel drug delivery systems, implantable devices, such as scaffoldds for cartilage regeneration and nanotechnology. Water soluble thermosensitive GP was developed as an injectable scaffold using EDC/NHS as a coupling reagent for catilage regeneration. $^1H$ NMR, and FTIR were used to characterize the chemical structure of the modified gelatins. The GP solution showed a reversible sol-gel transition behavior around body temperature. The cell viability was evaluated using methylthiazolyldiphenyl tetrazolium bromide (MTT) assay. The optical density (O.D.) of GP was significantly higher than that of $Pluronic^{(R)}$ at 1, 3 and 5 days, indicating that the cell viability on GP was better than on $Pluronic^{(R)}$. Therefore, the OP copolymer can be used as an injectable cell-containing scaffold owing to its thermally reversible properties and good biocompatibility.