The effects of monazite and fixing agents on cotton fabric dyed with Perilla frutescens var. acuta extract were investigated. The proper monazite treatment concentration, temperature and time were 10%(o.w.b.), $50^{circ}C$ and 60minutes. By various fixing agent treatment, $FeSO_4$ showed a relatively high K/S value and the order of K/S value decreased as follows, cation surface active agents, soybean and NaCl. And the monazite and $FeSO_4$ fixing agent showed higher anion emissity than those of untreated cotton and other fixing agents. The cotton fabrics showed improved color fastness by monazite and fixing agents treatments with the exception of light fastness. And the cotton fabrics fixed with fixing agents were showed effective bacterial reduction with the exception of NaCl.